By Corinne Whitby, Torben Lund Skovhus
Applied Microbiology and Molecular Biology in Oil box structures addresses the main difficulties microbes reason in oil fields, (e.g. biocorrosion and souring) and the way valuable microbial actions should be exploited (e.g. MEOR and biofuels). The publication describes theoretical and useful techniques to precise Molecular Microbiological tools (MMM), and is written by means of best experts within the box from either academia and undefined. The e-book describes how MMM will be utilized to faciliate larger administration of oil reservoirs and downstream approaches. The ebook is cutting edge in that it utilises genuine business case stories which provides precious technical and medical info to researchers, engineers and microbiologists operating with oil, fuel and petroleum systems.
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Additional resources for Applied Microbiology and Molecular Biology in Oilfield Systems: Proceedings from the International Symposium on Applied Microbiology and Molecular Biology in Oil Systems (ISMOS-2), 2009
1) and can be employed if one intends to characterise or enumerate the general community of microorganisms in a sample using the 16S rRNA gene as a target. , 2003). It is also possible to design primers/probes that target the variable and hypervariable regions of the 16S rRNA gene. This facilitates the development of groupspecific, genus-specific, and species-specific primers/probes. Such specific primers allow the amplification of the 16S rRNA gene from a small selection of closely related microorganisms.
This facilitates the development of groupspecific, genus-specific, and species-specific primers/probes. Such specific primers allow the amplification of the 16S rRNA gene from a small selection of closely related microorganisms. In the case of group-, genus-, and species-specific primers and probes, it is important to note that although the organisms in a phylogenetic group contain relatively similar 16S rRNA molecules, they may be functionally highly diverse. For example, the phylogenetic group Deltaproteobacteria contain sulphate- and sulphur-reducing, nitrate-utilising, aerobic, and iron-reducing microorganisms.
Ribosomal-RNA and messenger-RNA) provides insight in the active part of the total microbial population present. RNA is single stranded and subsequently more unstable than DNA. The RNA is therefore first ‘copied’ to DNA, creating cDNA (a DNA version of the RNA molecule, containing the code of the original RNA molecule) using an enzyme called reverse transcriptase. These cDNA gene fragments then can be analysed with DGGE, similar to a common DNA analysis, as described here. g. pollution with heavy metals, oil).
Applied Microbiology and Molecular Biology in Oilfield Systems: Proceedings from the International Symposium on Applied Microbiology and Molecular Biology in Oil Systems (ISMOS-2), 2009 by Corinne Whitby, Torben Lund Skovhus